کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5545908 1555644 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
T lymphocyte immunophenotypes in the cerebrospinal fluid of dogs with visceral leishmaniasis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
T lymphocyte immunophenotypes in the cerebrospinal fluid of dogs with visceral leishmaniasis
چکیده انگلیسی


- We correlated the T cells of the cerebrospinal fluid with the brain inflammation.
- Dogs with visceral leishmaniasis have similar amounts of T cells in blood and CSF.
- This study provides evidence of breakdown of the brain barriers.
- Double negative T cells may be contributing to brain inflammation in infected dogs.

Visceral leishmaniasis (VL) is a disease causing several clinical manifestations in dogs, including neurological disorders. Nevertheless, there are few studies related to the evaluation of the brain alterations during VL. Evidences of the involvement of cerebral barriers in infected dogs was reported, including the presence of brain inflammatory infiltrate, with a predominance of CD3+ T cells. Therefore, the aim of this study was to determine the immunophenotypes of T lymphocytes in the cerebrospinal fluid (CSF), as well as in peripheral blood, and to correlate with brain alterations in dogs with VL. We detected elevated percentages of double negative (DN) and double positive (DP) T cells in the CSF, with a predominance of TCRαb. In the histopathological analysis, we observed a predominance of lymphoplasmacytic infiltrate, mainly in leptomeninges, ranging from mild to intense, and we observed a positive correlation between the intensity of inflammation in the subependymal area and the DN T cells of the CSF. Thus, the DN T cells seem be acting as villains of the immune system through pro-inflammatory mechanisms. Further, the proportion of the different population of CSF T cells did not differ from those observed in the blood, which provides us with more evidence of blood-CSF barrier breakdown. Together, the results provide more explanation to the inflammation observed in the brain of dogs with VL, which the DN T cells contribute to the origin and progression of the neurological disease. This study provides insight into the immunophenotypes of T lymphocytes in the CSF during canine visceral leishmaniasis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 232, 15 December 2016, Pages 12-20
نویسندگان
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