Pharmacokinetic evaluation of Shenfu Injection in beagle dogs after intravenous drip administration

Shenfu Injection (SFI) is a well-defined Chinese herbal formulation that is obtained from red ginseng and processed aconite root. The main active constituents in SFI are ginsenosides and aconitum alkaloids. In this work, ginsenosides (ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rc) and aconitum alkaloids (benzoylmesaconine and fuziline) were used as the index components to explore the pharmacokinetic behavior of SFI. A selective and sensitive HPLC-MS/MS method was developed for the quantification of ginsenosides and aconitum alkaloids in dog plasma and was used to characterize the pharmacokinetics of the five index components after intravenous drip of three different dosages of SFI in beagle dogs. The pharmacokinetic properties of the index components were linear over the dose range of 2-8 mL/kg.

Shenfu Injection (SFI) is a commonly used traditional Chinese formulation composed of the extracts of red ginseng and aconite. Two alkaloids (benzoylmesaconine and fuziline) and three ginsenosides (Rg1, Rb1 and Rc), which are the main active ingredients, were selected as the indexes to explore the pharmacokinetics of SFI. Based on this, predictable and linear increase of the systemic exposure of SFI can be expected.