کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5553407 | 1557958 | 2016 | 7 صفحه PDF | دانلود رایگان |
BackgroundPresent study was designed to investigate the in vitro effects of nitric oxide (NO) donor such as S-nitroso-N-acetyl penicillamine (SNAP) on spontaneous exit from metaphase-II arrest (SEM-II) in rat eggs cultured in vitro.MethodsOvulated eggs were denuded and then exposed to various concentrations (0.0, 0.01, 0.1 and 1.0Â mM) of SNAP for 3Â h under in vitro culture conditions. The percentage of SEM-II, specific and total phosphorylated cyclin-dependent kinase-1 (Cdk1), cyclin B1 and anaphase promoting complex/cyclosome (APC/C) levels as well as Cdk1 activity were analyzed.ResultsThe SEM-II was associated with a decrease of Thr-161 phosphorylated Cdk1 as well as cyclin B1 levels and increase of Thr-14/Tyr-15 phosphorylated Cdk1, APC/C levels and Cdk1 activity in aged eggs cultured in vitro. On the other hand, SNAP treatment prevented a decrease of Thr-161 phosphorylated Cdk1 as well as cyclin B1 levels and increase of Thr-14/Tyr-15 phosphorylated Cdk1, Cdk1 activity that finally prevented SEM-II in a concentration-dependent manner. However, APC/C level was not affected by SNAP during the course of treatment in vitro.ConclusionsPresent data suggest that SNAP prevented SEM-II possibly by increasing high level of NO and thereby maturation promoting factor (MPF) stabilization in rat eggs cultured in vitro. Hence, SNAP could be used to prevent SEM-II that reduces reproductive outcome in several mammalian species.
121
Journal: Biomedicine & Pharmacotherapy - Volume 84, December 2016, Pages 680-686