کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5553585 1557956 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ghrelin upregulates PepT1 activity in the small intestine epithelium of rats with sepsis
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی تومور شناسی
پیش نمایش صفحه اول مقاله
Ghrelin upregulates PepT1 activity in the small intestine epithelium of rats with sepsis
چکیده انگلیسی


- Reveal the effect of ghrelin on the survival rate of rats with sepsis.
- Confirmed the relationship between ghrelin treatment and inflammatory response in intestinal epithelial cells of rats with sepsis.
- Found that ghrelin treatment contributes to the physiological role of PepT1 in intestinal epithelial cells of rats with sepsis.

BackgroundSepsis causes nutritional substrate malabsorption; hence, preventing gut barrier problems and improving the nutritional status in sepsis is a compelling issue.AimsWe tested whether ghrelin administration affects peptide transporter 1 (PepT1) activity in the intestinal epithelium of rats with sepsis.MethodsSixty male Sprague-Dawley rats were randomly divided into sham-operated, sepsis, and ghrelin-treated groups. The cecum of sham-operated rats was separated after laparotomy without ligation and perforation. Sepsis group rats underwent cecal ligation and puncture (CLP). Mucosal specimens were used for immunohistochemstry, real-time PCR, and western blotting to detect PepT1 distribution, and mRNA and protein expression levels, respectively. TNF-α, IL-1β, and ghrelin levels were estimated in serum and intestinal mucosal tissue by ELISA. High-performance liquid chromatography was used to measure PepT1 uptake by the epithelial cells. Moreover, survival, body weight, and food intake of the rats were recorded during the 7-day treatment period.ResultsAll rats in the sham-operated group survived, and 80% of rats in the sepsis group died within 7d of CLP. Treatment with ghrelin attenuated the CLP-induced body weight loss, intestine mucosa damage, and the survival rate was better. In addition, ghrelin attenuated increases in TNF-α and IL-1β production. The expressions of PepT1 mRNA and protein were higher in ghrelin-treated group rats than in sepsis rats. Moreover, the uptake function of PepT1 was better in ghrelin-treated group rats.ConclusionGhrelin treatment can reduce the inflammatory response and greatly upregulate the physiological function of PepT1 in intestinal epithelial cells of rats with sepsis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 86, February 2017, Pages 669-676
نویسندگان
, , , , , , , , ,