|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5558379||1561134||2017||10 صفحه PDF||سفارش دهید||دانلود کنید|
- Allergen and air pollutant exposures alter the immune profile of infant monkeys.
- Exposure effects were observed for CCR3, FoxP3 and IL-12 mRNA in peripheral blood.
- The lung immune profile following exposure was not comparable to peripheral blood.
Early life is a critical period for the progressive establishment of immunity in response to environmental stimuli; the impact of airborne challenges on this process is not well defined. In a longitudinal fashion, we determined the effect of episodic house dust mite (HDM) aerosol and ozone inhalation, both separately and combined, on peripheral blood immune cell phenotypes and cytokine expression from 4 to 25Â weeks of age in an infant rhesus monkey model of childhood development. Immune profiles in peripheral blood were compared with lung lavage at 25Â weeks of age. Independent of exposure, peripheral blood cell counts fluctuated with chronologic age of animals, while IFNÎ³ and IL-4 mRNA levels increased over time in a linear fashion. At 12Â weeks of age, total WBC, lymphocyte numbers, FoxP3 mRNA and IL-12 mRNA were dramatically reduced relative to earlier time points, but increased to a steady state with age. Exposure effects were observed for monocyte numbers, as well as CCR3, FoxP3, and IL-12 mRNA levels in peripheral blood. Significant differences in cell surface marker and cytokine expression were detected following in vitro HDM or PMA/ionomycin stimulation of PBMC isolated from animals exposed to either HDM or ozone. Lavage revealed a mixed immune phenotype of FoxP3, IFNÎ³ and eosinophilia in association with combined HDM plus ozone exposure, which was not observed in blood. Collectively, our findings show that airborne challenges during postnatal development elicit measureable cell and cytokine changes in peripheral blood over time, but exposure-induced immune profiles are not mirrored in the lung.
Journal: Toxicology and Applied Pharmacology - Volume 328, 1 August 2017, Pages 60-69