کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5558689 | 1561190 | 2017 | 9 صفحه PDF | دانلود رایگان |
- UVB radiation (280-320Â nm) exposure and cellular damages assessment in vitro.
- Damage progression assessed immediately and 24Â h post exposure using cultured human cells with more prominent damages expressed 24Â h post exposure.
- Cytotoxicity assessment investigated mitochondria, lysosomes, cell membrane and, DNA damages.
- The research reported significant cellular and DNA damages in addition to upregulation and downregulation of various apoptotic proteins.
The focus of this research was on UVB radiation (280-320Â nm) responsible for cellular changes in skin of acute and chronically exposed individuals. This study investigated the acute cellular damages triggered by UVB exposure of cultured human fibroblasts and keratinocyte cells immediately and 24Â h post exposure in order to understand damage onset and progression. The study evaluated a number of cellular parameters including mitochondria, lysosomes, cell membrane, DNA damages as well as pro and anti-apoptotic protein expression levels. Cellular organelle damages were assessed by a battery of in vitro toxicological assays using MTS and Neutral red cytotoxicity assays. Cell membrane damages were also assessed by measuring lactate dehydrogenase (LDH) enzyme leakage from UVB exposed cells. Lastly DNA damages was assessed using the comet assay while protein expression was evaluated using Western Blot.In this study we reported in all our assay systems (MTS, NR and LDH) that cellular damages were UVB dose dependent with damages amplified 24Â h post exposure. Our results also indicated that incubation of exposed cells for a period of 24Â h increased the sensitivity of the assay systems used. The increased sensitivity in detecting early cytotoxic damages was manifested though organelle damage measurement at very low doses which were not manifested immediately post exposure. The data also indicated that HaCaT cells were most sensitive in detecting UVB triggered damages immediately and 24Â h post exposure using the MTS assay. We also established upregulation and downregulation of various apoptotic proteins at various time points post exposure. The presented data clearly indicated the need for a comprehensive assessment of UVB damages 4 and 24Â h post exposure due to the different assay sensitivities in addition to various signaling mechanisms activated at different time points post exposure.
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Journal: Toxicology Reports - Volume 4, 2017, Pages 441-449