کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5560506 | 1561875 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Smoker extracellular vesicles influence status of human bronchial epithelial cells
ترجمه فارسی عنوان
حشیش خارج سلولی دودی که وضعیت سلول های اپیتلیال برونش انسانی را تحت تاثیر قرار می دهد
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کلمات کلیدی
HMGA2CSEEZH2EVSIARC یا International Agency for Research on Cancer - آژانس بین المللی تحقیقات سرطانInternational Agency for Research on Cancer - آژانس بین المللی تحقیقات سرطانCOPD - بیماری مزمن انسدادی ریهChronic obstructive pulmonary disease - بیماری مزمن انسدادی ریهenhancer of zeste homolog 2 - تقویت کننده یا ژنتیک همولوگ 2Extracellular vesicles - حامل های خارج سلولیcigarette smoke extract - عصاره سیگار سیگار
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
Cigarette smoking is a habit that has spread all over the world and is a significant risk factor for many diseases including cardiovascular disease, chronic obstructive pulmonary disease (COPD), asthma and lung cancer. Evaluation and understanding of tobacco health effects are of major interest worldwide and answer to important societal concerns. Identification of new biomarkers of exposure to tobacco smoke potentially implicated in COPD or lung carcinogenesis would allow a better observation of tobacco exposed population, thanks to screening establishment at reversible stages of pathological processes. In this study, we questioned whether cigarette smoking alters miRNA profiles of Extracellular Vesicles (EVs) present in human Broncho Alveolar Lavages (BALs), which could affect surrounding normal bronchial epithelial cells status. To this aim, BALs were carried out on 10 Smokers and 10 Non-Smokers, and EVs were isolated from the supernatants and characterized. We then compared the amount of 10 microRNAs (miRNAs) present in Smokers versus Non-Smokers BAL EVs and performed statistical analysis to discuss the biological significance by the smoking status and to evaluate BAL EV miRNAs as potential biomarkers of tobacco exposure. Finally, we tested the effects of smokers versus non-smokers EVs on human bronchial epithelial cells (BEAS-2B) to compare their influence on the cells status. Our study shows for the first time in human samples that smoking can alter lung EV profile that can influence surrounding bronchial epithelial cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Hygiene and Environmental Health - Volume 220, Issue 2, Part B, April 2017, Pages 445-454
Journal: International Journal of Hygiene and Environmental Health - Volume 220, Issue 2, Part B, April 2017, Pages 445-454
نویسندگان
Amélie Héliot, Yann Landkocz, Françoise Roy Saint-Georges, Pierre Gosset, Sylvain Billet, Pirouz Shirali, Dominique Courcot, Perrine J. Martin,