کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5561547 1562147 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Satellite rats are redundant in embryo-fetal development studies
ترجمه فارسی عنوان
موش های صحرایی در مطالعات توسعه جنین و جنین اضافی هستند
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


- Routinely, TK satellite rats are added to embryo-fetal development (EFD) studies.
- Microsampling eliminates use of satellite rats, but is not always possible.
- In those cases, jugular vein blood sampling would be a good alternative.
- This was shown by an exploratory EFD rat study with no treatment-related findings.
- In future, satellite rats can be omitted which reduces the animal usage by 20%.

Routinely in many laboratories, satellite rats are added to embryo-fetal development (EFD) studies for pharmaceuticals to assess toxicokinetic (TK) properties, because it is assumed that collection of multiple blood samples with relatively large volumes might affect the study outcome. With recent refinement of blood sampling techniques, this belief requires reevaluation. The current work showed successful implementation of jugular vein blood sampling in an EFD rat study without satellite animals, thereby reducing the number of rats in standard EFD studies for pharmaceuticals by 20%. Although not evaluated in this study, microsampling has shown to be very successful and eliminates the need of satellite animals. However, currently not all laboratories have implemented this method and regularly the bioanalytical method is already developed with a limit of quantification that is insufficiently sensitive. Therefore in those cases, a quick win to omit satellite animals can be established by using jugular vein blood sampling.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 72, September 2017, Pages 122-128
نویسندگان
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