کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5562601 1403430 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bioluminescent enzyme inhibition-based assay to predict the potential toxicity of carbon nanomaterials
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Bioluminescent enzyme inhibition-based assay to predict the potential toxicity of carbon nanomaterials
چکیده انگلیسی


- Bioluminescent enzymatic assay for CNM potential toxicity was proposed.
- The assay predicts toxicity decreasing in the following order: MWCNT > SWCNT > C60HyFn.
- Soluble and immobilised enzymes differ qualitatively in CNM toxicity prediction.

A bioluminescent enzyme inhibition-based assay was applied to predict the potential toxicity of carbon nanomaterials (CNM) presented by single- and multi-walled nanotubes (SWCNT and MWCNT) and aqueous solutions of hydrated fullerene С60 (C60HyFn). This assay specifically detects the influence of substances on parameters of the soluble or immobilised coupled enzyme system of luminescent bacteria: NAD(P)Н:FMN-oxidoreductase + luciferase (Red + Luc). A protocol based on the optical properties of CNM for correcting the results of the bioluminescent assay was also developed. It was shown that the inhibitory activity of CNM on Red + Luc decreased in the following order: MWCNT > SWCNT > C60HyFn. The soluble enzyme system Red + Luc had high sensitivity to MWCNT and SWCNT, with values of the inhibition parameter IC50 equal to 0.012 and 0.16 mg/L, respectively. The immobilised enzyme system was more vulnerable to C60HyFn than its soluble form, with an IC50 equal to 1.4 mg/L. Due to its technical simplicity, rapid response time and high sensitivity, this bioluminescent method has the potential to be developed as a general enzyme inhibition-based assay for a wide variety of nanomaterials.

290

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 45, Part 1, December 2017, Pages 128-133
نویسندگان
, , , , , , , , ,