کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5562604 1403430 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a quantitative cytotoxicity assay using mouse lymphoma TK cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Development of a quantitative cytotoxicity assay using mouse lymphoma TK cells
چکیده انگلیسی


- Cytotoxicity tests for materials that have contact with tissues continue to evolve.
- Current assays are impacted by mechanical damage from test substances.
- A new method uses suspension mouse lymphoma L5178Y thymidine kinase +/− (TK) cells.
- Cytotoxicity is measured using a cell counter combined with Trypan Blue to show the number of live and dead cells.
- This method may allow early detection of toxic leachables by medical devices.

We describe a new, quantitative, cytotoxicity method using mouse lymphoma L5178Y thymidine kinase +/− (TK) suspension cells for medical device safety assessment that has equivalent sensitivity, and greater efficiency compared to the colony formation assay (CFA) using adherent V79-4 cells. Assays were carried out in 24-well plates. TK cells were seeded on concave side of six types of contact lenses and cell growth rate after 72 h determined using the Vi-Cell XR. Five of six contact lenses studied demonstrated the same results between the TK and the CFA methods. One lens showed a difference between the CFA and TK suspension cell methods (79% colony formation rate vs. 17% cell growth rate). Although colony size is not a CFA test criterion, colonies were smaller than control indicating a cytotoxic effect that is reflected in the TK suspension cell assay which measures cell number. The mouse lymphoma TK suspension cell assay performs as well as the CFA direct contact method with distinct advantages. This quantitative, automated method is not susceptible to mechanical damage from test materials, does not depend on cell adherence to test materials and represents a valuable tool to evaluate medical device cytotoxicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 45, Part 1, December 2017, Pages 172-180
نویسندگان
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