کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5562704 | 1562705 | 2017 | 10 صفحه PDF | دانلود رایگان |
- Toxicological interactions occurred in HepG2 cells exposed to AgNPÂ +Â metals.
- Interactions affected cell viability, metabolism, proliferation and efflux transport.
- Increase of ROS levels was an early response.
- AgNP modulates Hg, but not Cd, uptake.
- AgNPÂ +Â Cd was more toxic than AgNPÂ +Â Hg.
Toxicological interaction represents a challenge to toxicology, particularly for novel contaminants. There are no data whether silver nanoparticles (AgNPs), present in a wide variety of products, can interact and modulate the toxicity of ubiquitous contaminants, such as nonessential metals. In the current study, we investigated the toxicological interactions of AgNP (size = 1-2 nm; zeta potential = â 23 mV), cadmium and mercury in human hepatoma HepG2 cells. The results indicated that the co-exposures led to toxicological interactions, with AgNP + Cd being more toxic than AgNP + Hg. Early (2-4 h) increases of ROS (DCF assay) and mitochondrial O2â levels (Mitosox® assay) were observed in the cells co-exposed to AgNP + Cd/Hg, in comparison to control and individual contaminants, but the effect was partially reverted in AgNP + Hg at the end of 24 h-exposure. In addition, decreases of mitochondrial metabolism (MTT), cell viability (neutral red uptake assay), cell proliferation (crystal violet assay) and ABC-transporters activity (rhodamine accumulation assay) were also more pronounced in the co-exposure groups. Foremost, co-exposure to AgNP and metals potentiated cell death (mainly by necrosis) and Hg2 + (but not Cd2 +) intracellular levels (ICP-MS). Therefore, toxicological interactions seem to increase the toxicity of AgNP, cadmium and mercury.
Journal: Toxicology in Vitro - Volume 40, April 2017, Pages 134-143