کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5562726 1562705 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of chitosan-coated lipid-core nanocapsules on bovine sperm cells
ترجمه فارسی عنوان
اثرات نانوکپسولهای لیپیدهای حاوی کیتوزان بر روی سلولهای اسپرم گاو
کلمات کلیدی
نانوسیستم نانوکاپسول، کیتوزان، اسپرم، اسپرم سم شناسی،
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


- Lipid-core nanocapsules (LNC) were coated with chitosan (CS).
- Toxicity of LNC and LNC-CS after incubation with bovine sperm in different concentrations
- CASA parameters and flow cytometry assays were performed to assess LNC and LNC-CS effects.
- There is no toxicity from LNCs and LNC-CS above 40% (v/v) concentration.

Toxicology studies have a pivotal role for selection of new nanosystems. As lipid-core nanocapsules (LNC) rise as a potential system not only for drug delivery but also for immunotherapy and gene therapy, the demand for models of toxic screening increases, and sperm arises as a promising model due to the easiness to evaluate its viability parameters. LNCs were coated with chitosan, chitosan-coated lipid-core nanocapsules (LNC-CS), in order to modify the nanocapsule surface. We evaluated the toxicity of LNC and LNC-CS after incubation with bovine sperm in different concentrations (2.5%, 5%, 10%, 20%, 40% and 80%) (v/v) and periods of exposure (0 h and 1 h). CASA parameters and flow cytometry assays were performed to assess LNC and LNC-CS effects. The results corroborated with previous studies indicating that there is no toxicity from LNCs and LNC-CS below 40% (v/v) concentration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 40, April 2017, Pages 214-222
نویسندگان
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