کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589451 | 1569797 | 2018 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Uncharacterized ORF HUR1 influences the efficiency of non-homologous end-joining repair in Saccharomyces cerevisiae
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کلمات کلیدی
PPINHEJSGADSBsDNA double stranded breaksalt-NHEJc-NHEJDDAMMEJsynthetic genetic array - آرایه مصنوعی ژنتیکیDNA repair - ترمیم DNAProtein-protein interaction - تعامل پروتئین-پروتئینGenetic interaction - تعامل ژنتیکیdouble-strand breaks - شکست دو ردیفnon-homologous end joining - عدم پیوستن انتهای غیر همولوگHomologous recombination - نوترکیبی همولوگHydroxyurea - هیدروکسی اوره
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Non-Homologous End Joining (NHEJ) is a highly conserved pathway that repairs Double-Strand Breaks (DSBs) within DNA. Here we show that the deletion of yeast uncharacterized ORF HUR1, Hydroxyurea Resistance1 affects the efficiency of NHEJ. Our findings are supported by Protein-Protein Interaction (PPI), genetic interaction and drug sensitivity analyses. To assess the activity of HUR1 in DSB repair, we deleted its non-overlapping region with PMR1, referred to as HUR1-A. We observed that similar to deletion of TPK1 and NEJ1, and unlike YKU70 (important for NHEJ of DNA with overhang and not blunt end), deletion of HUR1-A reduced the efficiency of NHEJ in both overhang and blunt end plasmid repair assays. Similarly, a chromosomal repair assay showed a reduction for repair efficiency when HUR1-A was deleted. In agreement with a functional connection for Hur1p with Tpk1p and NEJ1p, double mutant strains Îhur1-A/Îtpk1, and Îhur1-A/Înej1 showed the same reduction in the efficiency of plasmid repair, compared to both single deletion strains. Also, using a Homologous Recombination (HR) specific plasmid-based DSB repair assay we observed that deletion of HUR1-A influenced the efficiency of HR repair, suggesting that HUR1 might also play additional roles in other DNA repair pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 639, 10 January 2018, Pages 128-136
Journal: Gene - Volume 639, 10 January 2018, Pages 128-136
نویسندگان
Katayoun Omidi, Matthew Jessulat, Mohsen Hooshyar, Daniel Burnside, Andrew Schoenrock, Tom Kazmirchuk, Maryam Hajikarimlou, Mary Daniel, Houman Moteshareie, Urvi Bhojoo, Megan Sanders, Dindial Ramotar, Frank Dehne, Bahram Samanfar, Mohan Babu,