کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589511 | 1569800 | 2017 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MicroRNA profiles of fibroblasts derived from in vivo fertilized and fat-1 transgenic cattle
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کلمات کلیدی
TGF-βmiRNAshBD-3FFAIn vivo fertilizationFgf10Atgl - اتگلFree fatty acids - اسیدهای چرب آِزادMOET - بایدtransforming growth factor-β - تبدیل فاکتور رشد βmultiple ovulation and embryo transfer - تخمک گذاری چند و انتقال جنینTransgenic - تراریختهTransgenesis - ترانس ژنزKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوmicroRNAs - ریز آرانایfibroblast growth factor 10 - عامل رشد فیبروبلاست 10phosphatase and tensin homolog - فسفاتاز و تنسین همولوگFibroblasts - فیبروبلاست هاadipose triglyceride lipase - لیپاز تری گلیسیرید چربیMicroRNA - میکرو RNA Fat-1 - چربی 1Pten - ژن PTEN
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Fat-1 transgenic cattle have high levels of Ï-3 fatty acids, which regulate several genes in fatty acid metabolism. In the current study, fibroblasts derived from in vivo fertilized (Ferti) and fat-1 transgenic (TG) Luxi cattle (Bos taurus), a local breed in China, were cultured and their miRNA expression was characterized. Expression of 352 known miRNAs differed in cells from Ferti and TG cattle: 83 miRNAs were found to be specifically expressed in cells from Ferti cattle while 23 miRNAs were found to be specifically expressed in cells from TG cattle. Novel differences in miRNA expression were also found in cells from Ferti and TG cattle. The identity of seven differentially expressed miRNAs was verified using quantitative real-time PCR, and target genes were identified computationally. GO and KEGG analysis revealed that these miRNAs were involved in seven major biological pathways, including metabolism, MAPK signaling, calcium signaling, purine metabolism, ubiquitin mediated proteolysis, pyrimidine metabolism, and the cell cycle. Overexpression of one of these miRNAs, miR-21-5p, was found to suppress expression of fibroblast growth factor 10 (FGF10) and adipose triglyceride lipase (ATGL) in fibroblasts from TG cattle and 3T3-L1 pre-adipocytes. Conversely, knockdown of miR-21-5p stimulated expression. Together, these results suggest that miRNAs potentially play a role in expression of lipogenic and lipolytic genes as well as in synthesis of Ï-3 fatty acids facilitated by fat-1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 636, 15 December 2017, Pages 70-77
Journal: Gene - Volume 636, 15 December 2017, Pages 70-77
نویسندگان
Yang Lv, Yu Wang, Jiajia Sun, Chunling Gong, Yan Chen, Guanghua Su, Guangqi Gao, Chunling Bai, Zhuying Wei, Lisheng Zhang, Shorgan Bou, Guangpeng Li,