کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589515 | 1569800 | 2017 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Counter regulation of ECRG4 gene expression by hypermethylation-dependent inhibition and the Sp1 transcription factor-dependent stimulation of the c2orf40 promoter
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کلمات کلیدی
5-azaCEGFRGSPRPMIHUVECPMNHypermethylation5-AzacytidineORFGFAPTISPBMCACTHDMEMspecific protein-1transcription initiation sitesDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoSp1 - SP1Gene-specific primer - آغازگر مخصوص ژنEDTA - اتیلن دی آمین تترا استیک اسید Ethylene diamine tetra acetic acid - اتیلن دیامین تترا استیک اسیدribonucleic acid - اسید ریبونوکلئیکRNA - اسید ریبونوکلئیکchromatin immunoprecipitation - ایمن سازی کروماتینrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدbase pair - جفت پایهreverse transcription - رونویسی معکوسHuman umbilical vein endothelial cell - سلول اندوتلیالی ورید ناقل انسانیPeripheral blood mononuclear cell - سلول تک هسته ای خون محیطیJurkat cells - سلولهای JurkatPolymorphonuclear cells - سلولهای پلیمورفونیکcytomegalovirus - سیتومگالوویروسCMV - سیتومگالوویروسnuclear factor - عامل هسته ایSp1 transcription factor - فاکتور رونویسی Sp1open reading frame - قاب خواندن بازluciferase - لوسیفرازRace - مسابقهRoswell Park Memorial Institute medium - موسسه خاطرات Roswell Park mediumpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالPromoter - پروموترChoroid plexus - پیوند کوئیدCHiP - چیپEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Counter regulation of ECRG4 gene expression by hypermethylation-dependent inhibition and the Sp1 transcription factor-dependent stimulation of the c2orf40 promoter Counter regulation of ECRG4 gene expression by hypermethylation-dependent inhibition and the Sp1 transcription factor-dependent stimulation of the c2orf40 promoter](/preview/png/5589515.png)
چکیده انگلیسی
The human cytokine precursor ECRG4 has been associated with multiple physiological, developmental and pathophysiological processes involving cell proliferation, cell migration, innate immunity, inflammation, cancer progression and metastases. Although down-regulation of ECRG4 gene expression has been largely attributed to hypermethylation of CpG islands in the 5'untranslated region of the ECRG4 promoter, the mechanisms that underlie the dynamics of its regulation have never been systematically described. Here we show that the ECRG4 gene is widely expressed in human tissues and report that its core promoter lies between the â 780 to + 420 base pairs relative to the ATG start codon of the ECRG4 open reading frame. This sequence, which contains several CpG islands, also includes multiple overlapping Sp1 consensus binding sequences and a putative binding site for NF-kB activation. 5â²RACE of mRNA derived from human leukocytes shows that ECRG4 transcription initiates from the guanidine at â 11 from the initiation ATG of the ECRG4 open reading frame. While there is no canonical TATA- or CAAT-boxes proximal to this translational initiation site, there is a distal TATA-sequence in the 5â²UTR. This region was identified as the sequence targeted by hypermethylation because in vitro methylation of plasmids encoding the ECRG4 promoter abolish promoter activity and the treatment of Jurkat cells (which naturally express ECRG4) with the methylation inhibitor 5-AzaC, increases endogenous ECRG4 expression. Because ChIP assays show that Sp1 binds the ECRG4 promoter, that forced Sp1 expression trans-activates the ECRG4 promoter and Sp1 inhibition with mithramycin inhibits ECRG4 expression, we conclude that the dynamic positive and negative regulatory elements controlling ECRG4 expression include a counter regulation between promoter methylation and Sp1 activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 636, 15 December 2017, Pages 103-111
Journal: Gene - Volume 636, 15 December 2017, Pages 103-111
نویسندگان
Xitong Dang, Xiaorong Zeng, Raul Coimbra, Brian P. Eliceiri, Andrew Baird,