کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589626 | 1569809 | 2017 | 46 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
greb1 regulates convergent extension movement and pituitary development in zebrafish
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
GTHHPGPRLTSHhpfRT-PCRGREB1PBSWISH - آرزو کردنEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاPituitary development - توسعه هیپوفیزbase pairs - جفت پایهGrowth - رشدTALEN - زبان هاReal time-polymerase chain reaction - زمان واقعی واکنش زنجیره ای پلیمرازhour post-fertilization - ساعت پس از لقاحphosphate buffer solution - محلول بافر فسفاتknockout - ناکاوتConvergence and extension - همگرایی و گسترشHormone - هورمونfollicle-stimulating hormone - هورمون تحریک کننده فولیکولluteinizing hormone - هورمون جسم زردGrowth hormone - هورمون رشدthyroid stimulating hormone - هورمون محرک تیروئیدFSH - هورمون محرکه فولیکولی Hypothalamic-pituitary-gonadal - هیپوتالاموس-هیپوفیز-گونادالProlactin - پرولاکتین Whole mount in situ hybridization - کل کوهنوردی در محل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
greb1, growth regulation by estrogen in breast cancer 1, is an important estrogen response gene, which participates in breast cancer formation. However, the role of greb1 in development is largely unknown. In this study, we have characterized greb1 expression patterns and revealed its regulative role in convergent extension movement and pituitary development in zebrafish. In zebrafish adults, greb1 was revealed to be expressed mainly in pituitary, hypothalamus, liver, and gonads. During embryogenesis, RT-PCR and WISH revealed its spatial and temporal expression specific to embryonic margin during gastrulation, central nervous system during segmentation and brain ventricular regions from 24Â hpf. To investigate its function, we utilized TALEN-mediated knockout strategy and obtained a greb1 mutant zebrafish line. greb1 mutation caused convergent extension (CE) movement defect and developmental delay during gastrulation, and resulted in significant growth defects in zebrafish adults. Moreover, four pituitary cells including somatotropes, thyrotropes, lactotropes and gonadotropes were specifically reduced during embryogenesis. Importantly, pituitary hormones-encoding genes and their serum products, such as GH, TSH, PRL, FSH, and LH, were severely diminished in greb1 mutants, and high mortality and significant growth defects were observed. Therefore, our present study indicates that greb1 is a novel regulator for CE movement and pituitary development in zebrafish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 627, 5 September 2017, Pages 176-187
Journal: Gene - Volume 627, 5 September 2017, Pages 176-187
نویسندگان
Shi-Zhu Li, Wei Liu, Zhi Li, Wen-Hua Li, Yang Wang, Li Zhou, Jian-Fang Gui,