کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589663 | 1569809 | 2017 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cinnamon extracts exert intrapancreatic cytoprotection against streptozotocin in vivo
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کلمات کلیدی
NF-κBcTMSIL-6T1DSTZGTTRT-PCRGlucose tolerance testing - آزمایش تحمل گلوکزstreptozotocin - استرپتوزوتوسینinflammation - التهاب( توروم) insulin - انسولینinterleukin-6 - اینترلوکین ۶Enzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاtumor necrosis factor-α - تومور نکروز عامل αType 1 diabetes - دیابت نوع۱TNF-α - فاکتور نکروز توموری آلفاpolymerase chain reaction - واکنش زنجیره ای پلیمرازPancreas - پانکراس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
In the current study, we aimed to investigate the potential hypoglycemic bioactivity of cinnamon extracts (CES) on streptozotocin (STZ)-induced hyperglycemia in mice. In biological methods, glucose metabolic ability of all mice was evaluated by glucose tolerance testing (GTT). Blood levels of pancreas-produced insulin, glucagon, inflammation-associated interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) were determined via enzyme-linked immunosorbent assay (ELISA). In gene level, intrapancreatic mRNA expressions of insulin, TNF-α and nuclear-factor kappa B (NF-κB) were assayed by reverse transcription polymerase chain reaction (RT-PCR). Meanwhile, intracellular insulin-immunoactive cells in the pancreas were analyzed by using immunohistochemical staining. In addition, the protein levels of intrapancreatic NF-κB, IκB (p-IκB) and IKK were tested by western blotting. As a result, CES-treated mice showed increased body weight, blood glucose and insulin, reduced IL-6 and TNF-α contents in sera. Further, the TNF-α and NF-κB mRNA expressions in the CES-treated pancreas were down-regulated at a dose-dependent manner, while insulin mRNA was elevated. Moreover, the reduced intrapancreatic NF-κB, IκB (p-IκB) and IKK expression were observed in CES-treated pancreas, respectively. Taken together, our current findings indicate that CES-mediated intrapancreatic cytoprotection is linked to the molecular mechanism that may be through inhibiting inflammatory stress and promoting insulin secretion in the pancreas.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 627, 5 September 2017, Pages 519-523
Journal: Gene - Volume 627, 5 September 2017, Pages 519-523
نویسندگان
Zuozhuang Liao, Jinni Wang, Hongdi Tan, Limei Wei,