کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589673 | 1569809 | 2017 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A cysteine protease (cathepsin Z) from disk abalone, Haliotis discus discus: Genomic characterization and transcriptional profiling during bacterial infections
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کلمات کلیدی
PBSCathepsin ZgDNACFUCTSZLPSp.iCysqPCRCDD - CSDgenomic DNA - DNA ژنومیBAC - LACBLAST, basic local alignment search tool - ابزار پایهای برای جستجوی برهم نهیهای موضعی، بلاستstandard deviation - انحراف معیارBlast - انفجارPost Injection - تزریق پستcoding sequence - توالی کدگذاریDisk abalone - دیسک شفافquantitative real time PCR - زمان واقعی PCR کمیGenomic organization - سازمان ژنومیCysteine - سیستئینPhosphate buffered saline - فسفات بافر شورlipopolysaccharide - لیپوپلی ساکاریدUntranslated regions - مناطق غیر ترجمهUTR یا untranslated regions - منطقه ترجمه نشدهcolony forming units - واحدهای تشکیل دهنده کلنیCdS - کادمیم سولفید، سولفید کادمیمbacterial artificial chromosome - کروموزوم مصنوعی باکتریایی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Cathepsin Z (CTSZ) is lysosomal cysteine protease of the papain superfamily. It participates in the host immune defense via phagocytosis, signal transduction, cell-cell communication, proliferation, and migration of immune cells such as monocytes, macrophages, and dendritic cells. Hence, CTSZ is also acknowledged as an acute-phase protein in host immunity. In this study, we sought to identify the CTSZ homolog from disk abalone (AbCTSZ) and characterize it at the molecular, genomic, and transcriptional levels. AbCTSZ encodes a protein with 318 amino acids and a molecular mass of 36Â kDa. The structure of AbCTSZ reveals amino acid sequences that are characteristic of the signal sequence, pro-peptide, peptidase-C1 papain family cysteine protease domain, mini-loop, HIP motif, N-linked glycosylation sites, active sites, and conserved Cys residues. A pairwise comparison revealed that AbCTSZ shared the highest amino acid homology with its molluscan counterpart from Crassostrea gigas. A multiple alignment analysis revealed the conservation of functionally crucial elements of AbCTSZ, and a phylogenetic study further confirmed a proximal evolutionary relationship with its invertebrate counterparts. Further, an analysis of AbCTSZ genomic structure revealed seven exons separated by six introns, which differs from that of its vertebrate counterparts. Quantitative real time PCR (qPCR) detected the transcripts of AbCTSZ in early developmental stages and in eight different tissues. Higher levels of AbCTSZ transcripts were found in trochophore, gill, and hemocytes, highlighting its importance in the early development and immunity of disk abalone. In addition, we found that viable bacteria (Vibrio parahaemolyticus and Listeria monocytogenes) and bacterial lipopolysaccharides significantly modulated AbCTSZ transcription. Collectively, these lines of evidences suggest that AbCTSZ plays an indispensable role in the innate immunity of disk abalone.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 627, 5 September 2017, Pages 500-507
Journal: Gene - Volume 627, 5 September 2017, Pages 500-507
نویسندگان
G.I. Godahewa, N.C.N. Perera, Sukkyoung Lee, Myoung-Jin Kim, Jehee Lee,