کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5590423 | 1570152 | 2017 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
De novo transcriptome sequencing and analysis of the juvenile and adult stages of Fasciola gigantica
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کلمات کلیدی
SNPsNCBIKOGSSRRPKMFasciola giganticareads per kb per million reads - به ازای هر میلیون نسخه خوانده می شودde novo transcriptome - ترنسپتوم جدیدHost-pathogen interaction - تعامل میزبان-پاتوژنNext-generation sequencing - تعیین توالی نسل بعدیKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوsimple sequence repeat - دنباله ساده تکرار کنیدNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیGene ontology - هستیشناسی ژنیnon-redundant protein database - پایگاه داده پروتئین غیر انحصاریSingle nucleotide polymorphisms - پلیمورفیسم تک نوکلئوتیدیDifferentially expressed genes - ژن های متفاوت بیان شده است
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
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چکیده انگلیسی
Fasciola gigantica is regarded as the major liver fluke causing fasciolosis in livestock in tropical countries. Despite the significant economic and public health impacts of F. gigantica there are few studies on the pathogenesis of this parasite and our understanding is further limited by the lack of genome and transcriptome information. In this study, de novo Illumina RNA sequencing (RNA-seq) was performed to obtain a comprehensive transcriptome profile of the juvenile (42 days post infection) and adult stages of F. gigantica. A total of 49,720 unigenes were produced from juvenile and adult stages of F. gigantica, with an average length of 1286 nucleotides (nt) and N50 of 2076 nt. A total of 27,862 (56.03%) unigenes were annotated by BLAST similarity searches against the NCBI non-redundant protein database. Because F. gigantica needs to feed and/or digest host tissues, some proteases (including cysteine proteases and aspartic proteases), which play a role in the degradation of host tissues (protein), have been paid more attention in the present study. A total of 6511 distinct genes were found differentially expressed between juveniles and adults, of which 3993 genes were up-regulated and 2518 genes were down-regulated in adults versus juveniles, respectively. Moreover, stage-specific differentially expressed genes were identified in juvenile (17,009) and adult (6517) F. gigantica. The significantly divergent pathways of differentially expressed genes included cAMP signaling pathway (226; 4.12%), proteoglycans in cancer (256; 4.67%) and focal adhesion (199; 3.63%). The transcription pattern also revealed two egg-laying-associated pathways: cGMP-PKG signaling pathway and TGF-β signaling pathway. This study provides the first comparative transcriptomic data concerning juvenile and adult stages of F. gigantica that will be of great value for future research efforts into understanding parasite pathogenesis and developing vaccines against this important parasite.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Infection, Genetics and Evolution - Volume 51, July 2017, Pages 33-40
Journal: Infection, Genetics and Evolution - Volume 51, July 2017, Pages 33-40
نویسندگان
Xiao-Xuan Zhang, Wei Cong, Hany M. Elsheikha, Guo-Hua Liu, Jian-Gang Ma, Wei-Yi Huang, Quan Zhao, Xing-Quan Zhu,