کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5591564 | 1404982 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The pH-dependent assembly of Chaplin E from Streptomyces coelicolor
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کلمات کلیدی
SRCDS. coelicolorStreptomyces coelicolorACNDLSTFAIEFdichlorodiphenyltrichloroethaneAcetonitrile - استونیتریلTrifluoracetic acid - اسید TrifluoraceticTem - این استThT - بلهFourier transform infra red - تبدیل فوریه مادون قرمزThioflavin T - تیوفلاوین TDDT - دیکرو دیفنیل تری کلرواتانcircular dichroism - رنگ تابی دورانیMolecular dynamics simulations - شبیه سازی پویایی مولکولیFTIR - طیف سنج مادون قرمزhydrodynamic diameter - قطر هیدرودینامیکیTransmission electron microscopy - میکروسکوپ الکترونی عبوریIsoelectric point - نقطه ایزوالکتریکDynamic Light Scattering - پراکندگی نور دینامیکی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The pH-dependent assembly of Chaplin E from Streptomyces coelicolor The pH-dependent assembly of Chaplin E from Streptomyces coelicolor](/preview/png/5591564.png)
چکیده انگلیسی
Chaplin E, is one of five self-assembling peptides secreted by Streptomyces coelicolor that assist aerial growth by lowering the surface tension of water. Although the surface activity of a mixture of chaplin peptides has observed to depend on pH, it is unclear how the solvent environment (i.e. pH) influences the structure, assembly and subsequent functionality of these individual peptides. In this study, the conformation and fibril forming propensity of the Chaplin E peptide was assessed as a function of pH using a combination of experimental measurements and molecular dynamics simulations. At an acidic pH of 3.0, Chaplin E retained a random coil structure, whereas at the isoelectric point of 6.7 or a basic pH of 10.0, Chaplin E rapidly formed amyloid fibrils rich in β-sheet structure with high efficiency (>93%). Molecular dynamics simulations indicate the persistence of greater α-helical content at the N-terminus at high pH; this is likely partly due to the lack of electrostatic repulsion between residues His6 and Lys10. Since fibril formation was observed at high but not at low pH, we propose that the presence of an N-terminal α-helix in the monomeric form of Chaplin E is required for aggregation and conversion to β-amyloid fibrils. The pH sensitivity of Chaplin E peptide structure provides a route to control peptide assembly and may be important for the physiological function of this peptide, as a surface active agent in the transition from vegetative to aerial growth and could assist Streptomyces coelicolor in response to environmental fluctuations in pH.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 198, Issue 2, May 2017, Pages 82-91
Journal: Journal of Structural Biology - Volume 198, Issue 2, May 2017, Pages 82-91
نویسندگان
Mina Dokouhaki, Andrew Hung, Li Day, Sally L. Gras,