کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5591859 | 1570702 | 2017 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
HLA-E regulatory and coding region variability and haplotypes in a Brazilian population sample
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کلمات کلیدی
HCMVHsp60MAFTCrNGSmRNAVCFInfluenza matrix proteinPRDX5IMGTDNA - DNA یا اسید دزوکسی ریبونوکلئیکmessenger RNA - RNA messengerHuman leukocyte antigen - آنتی ژن لوسکسی انسانHLA - آنتیژن گلبول سفید انسانیSINE - آنهاdeoxyribonucleic acid - اسید deoxyribonucleicEBV - اپشتین بار ویروسVariability - تغییرپذیریNext generation sequencing - توالی نسل بعدیbase pairs - جفت پایهNatural killer cell - سلول قاتل طبیعیHuman cytomegalovirus - سیتومگالوویروس انسانیShort interspersed element - عنصر مبهم کوتاهminor allele frequency - فراوانی آللی جزئیvariant call format - فرمت تماس نوعیMHC - مجموعه سازگاری بافتی اصلیmajor histocompatibility complex - مجموعه سازگاری بافتی اصلیUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهHCV - هپاتیت سیHepatitis C virus - هپاتیت سیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازEpstein-Barr virus - ویروس Epstein-BarrHIV - ویروس نقص ایمنی انسانی human immunodeficiency virus - ویروس نقص ایمنی انسانیHerpes virus - ویروس هرپسHPV - ویروس پایپلوم انسانیPeroxiredoxin 5 - پراکسی اوردوکسین 5heat shock protein 60 - پروتئین شوک حرارت 60Promoter - پروموترPolymorphism - پلی مورفیسمSingle nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیSNP - چندریختی تک-نوکلئوتیدT cell receptor - گیرنده سلول T
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
The HLA-E gene is characterized by low but wide expression on different tissues. HLA-E is considered a conserved gene, being one of the least polymorphic class I HLA genes. The HLA-E molecule interacts with Natural Killer cell receptors and T lymphocytes receptors, and might activate or inhibit immune responses depending on the peptide associated with HLA-E and with which receptors HLA-E interacts to. Variable sites within the HLA-E regulatory and coding segments may influence the gene function by modifying its expression pattern or encoded molecule, thus, influencing its interaction with receptors and the peptide. Here we propose an approach to evaluate the gene structure, haplotype pattern and the complete HLA-E variability, including regulatory (promoter and 3â²UTR) and coding segments (with introns), by using massively parallel sequencing. We investigated the variability of 420 samples from a very admixed population such as Brazilians by using this approach. Considering a segment of about 7Â kb, 63 variable sites were detected, arranged into 75 extended haplotypes. We detected 37 different promoter sequences (but few frequent ones), 27 different coding sequences (15 representing new HLA-E alleles) and 12 haplotypes at the 3â²UTR segment, two of them presenting a summed frequency of 90%. Despite the number of coding alleles, they encode mainly two different full-length molecules, known as E*01:01 and E*01:03, which corresponds to about 90% of all. In addition, differently from what has been previously observed for other non classical HLA genes, the relationship among the HLA-E promoter, coding and 3â²UTR haplotypes is not straightforward because the same promoter and 3â²UTR haplotypes were many times associated with different HLA-E coding haplotypes. This data reinforces the presence of only two main full-length HLA-E molecules encoded by the many HLA-E alleles detected in our population sample. In addition, this data does indicate that the distal HLA-E promoter is by far the most variable segment. Further analyses involving the binding of transcription factors and non-coding RNAs, as well as the HLA-E expression in different tissues, are necessary to evaluate whether these variable sites at regulatory segments (or even at the coding sequence) may influence the gene expression profile.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 91, November 2017, Pages 173-184
Journal: Molecular Immunology - Volume 91, November 2017, Pages 173-184
نویسندگان
Jaqueline Ramalho, Luciana C. Veiga-Castelli, Eduardo A. Donadi, Celso T. Mendes-Junior, Erick C. Castelli,