کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5592195 | 1570713 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunogenic peptides: B & T Cell Epitopes of Per a 10 Allergen of Periplaneta americana
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کلمات کلیدی
PBMCIgEADHNHSSDAPB cell epitopesHuman leukocyte antigen - آنتی ژن لوسکسی انسانHLA - آنتیژن گلبول سفید انسانیAlcohol dehydrogenase - الکل دهیدروژنازdiscrete optimized protein energy - انرژی پروتئینی بهینه شده به صورت جداگانهT cell epitopes - اپیتوپ های T سلولیIn silico - درون رایانهای، این سیلیکوPeripheral blood mononuclear cell - سلول تک هسته ای خون محیطیPeptides - پپتیدهاDOPE - پیش بینی کردن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Immunogenic peptides: B & T Cell Epitopes of Per a 10 Allergen of Periplaneta americana Immunogenic peptides: B & T Cell Epitopes of Per a 10 Allergen of Periplaneta americana](/preview/png/5592195.png)
چکیده انگلیسی
Mapping of B and T cell epitopes of an allergen can be utilised in the development of alternative therapeutic modalities and diagnostics. The present study was aimed to identify B and T cell epitopes of Per a 10, a major cockroach allergen, by computational tools and subsequent validation by in vitro experiments. Per a 10 three-dimensional structure was homology modelled using structure of anionic trypsin from pacific chum salmon as a template. Seven B cell epitopes (B-P1 to B-P7) were predicted by sequence and structure based methods. Three T cell epitopes (T-P8 to T-P10) were predicted by binding score and inhibitory concentration dependent prediction tools. Predicted epitopes were synthesized and biological activity was assessed by ELISA, ELISA inhibition and PBMC proliferation assays. B cell peptides B-P5, B-P6 and B-P7 showed significantly high IgE binding with pooled and individual cockroach hypersensitive patients' sera while the T cell peptides did not show IgE binding. ELISA inhibition was performed to determine the potency of the predicted peptides. Fifty nanogram of peptide B-P7 was required for 50% IgE binding inhibition of surface bound Per a 10 whereas seventy five nanogram and ninety nanogram of B-P5 and B-P6 were required for the same respectively. Upon stimulation with T-P8 and T-P10 peptides, PBMCs from cockroach allergic patients' (nâ=â15) showed significant lymphocyte proliferation and induced IL-4 and IL-5 cytokine release in the culture supernatant demonstrating Th2 dominant cell mediated response of predicted T cell peptides. In conclusion, Per a 10 3-D structure obtained by homology modelling was used to identify B and T cell epitopes, followed by in vitro validation. The identified peptides can be potentially used in designing diagnostics and therapies for cockroach allergy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 80, December 2016, Pages 24-32
Journal: Molecular Immunology - Volume 80, December 2016, Pages 24-32
نویسندگان
Dhanapal Govindaraj, Swati Sharma, S.N. Gaur, Shakuntala Lavasa, Nagendra Prasad, Naveen Ph.D,