کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5664648 1407716 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of hypoxia preconditioning on the neural and stemness genes expression profiling in human umbilical cord blood mesenchymal stem cells
ترجمه فارسی عنوان
تاثیر پیش تنظیم هیپوکسی بر پروفایل بیان ژن های عصبی و ساقه در سلول های بنیادی مزانشیمی خون بند ناف
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی هماتولوژی
چکیده انگلیسی
In recent years, human umbilical cord blood-derived mesenchymal stem cell (hUB-MSCs) has been regarded as an alternative source for stem cell therapy. In this study, we evaluated the effect of hypoxia preconditioning (HPC) on the expression of Nt-3, GFAP, Nestin, Oct-4 and Nanog genes and proliferative capacity of hUB-MSCs in comparison with normoxic conditions. HPC + Hypoxia protocol includes cultured hUB-MSCs for 15 min at 2.5% O2 and after that reoxygenation for 30 min at 21% O2 (HPC), and then hypoxia preconditioned hUB-MSCs subjected to 2.5% O2 for 72 h (Hypoxia). Conclusively, the results showed that hypoxic preconditioning is an effective strategy for enhancing proliferation capacity of hUB-MSCs, and also can trigger expression of some of the neural genes. In addition, the concept of involvement of oxygen tension in the expression of some of the neural genes of hUB-MSCs would be a good sign of enhanced neural differentiation potential in vitro.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transfusion and Apheresis Science - Volume 56, Issue 3, June 2017, Pages 392-399
نویسندگان
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