کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5666797 1591737 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hyperbaric oxygen therapy augments tobramycin efficacy in experimental Staphylococcus aureus endocarditis
ترجمه فارسی عنوان
اکسیژن درمانی بیش از حد با افزایش ترومبایسین در استافیلوکوکوس اورئوس آندوکاردیت
کلمات کلیدی
اکسیژن درمانی بیش از حد، پاسخ میزبان، بیوفیلم، هیپوکسیا، نوتروفیل ها، استرس اکسیداتیو،
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی


- Staphylococcus aureus infective endocarditis (IE) is a serious acute infection with reported mortality of up to 40%.
- Proposed oxygen-dependent bactericidal effect of aminoglycosides.
- Hyperbaric oxygen therapy (HBOT) augments tobramycin efficacy in cardiac valve vegetations.
- Host response in experimental S. aureus IE was evaluated by key inflammatory markers.
- Proof of concept using adjunctive HBOT in severe S. aureus IE.

Staphylococcus aureus infective endocarditis (IE) is a serious disease with an in-hospital mortality of up to 40%. Improvements in the effects of antibiotics and host responses could potentially benefit outcomes. Hyperbaric oxygen therapy (HBOT) represents an adjunctive therapeutic option. In this study, the efficacy of HBOT in combination with tobramycin in S. aureus IE was evaluated. A rat model of S. aureus IE mimicking the bacterial load in humans was used. Infected rats treated subcutaneously with tobramycin were randomised into two groups: (i) HBOT twice daily (n = 13); or (ii) normobaric air breathing (non-HBOT) (n = 17). Quantitative bacteriology, cytokine expression, valve vegetation size and clinical status were assessed 4 days post-infection. Adjunctive HBOT reduced the bacterial load in the aortic valves, myocardium and spleen compared with the non-HBOT group (P = 0.004, <0.001 and 0.01, respectively) and improved the clinical score (P <0.0001). Photoplanimetric analysis and weight of valve vegetations showed significantly reduced vegetations in the HBOT group (P <0.001). Key pro-inflammatory cytokines [IL-1β, IL-6, keratinocyte-derived chemokine (KC) and vascular endothelial growth factor (VEGF)] were significantly reduced in valves from the HBOT group compared with the non-HBOT group. In conclusion, HBOT augmented tobramycin efficacy as assessed by several parameters. These findings suggest the potential use of adjunctive therapy in severe S. aureus IE.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Antimicrobial Agents - Volume 50, Issue 3, September 2017, Pages 406-412
نویسندگان
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