Life cycle of Spinose ear tick, Otobius megnini (Acari: Argasidae) infesting the race horses in Nuwara Eliya, Sri Lanka

- Otobius megnini completes its life cycle within 123 days.
- It has only one nymphal instar in the life cycle.
- Nymphs show diapause at low temperature.
- Some females laid eggs without mating.
- Females produce vibrations to attract males.

Otobius megnini infests mainly domesticated and wild animals and occasionally humans. It has been identified as an agent of human and horse otoacariasis in Sri Lanka. The number of nymphal stages in the life cycle of O. megnini varies from one to three in different geographic regions. The present study determined the life cycle of O. megnini infesting the racehorses in Nuwara Eliya. A tick colony was initiated from fully engorged nymphs collected from horses and maintained at 10 °C, 22 °C and 28 °C. Immature stages were reared on New Zealand rabbits. Only the larvae weighing more than 0.9 mg (84.9%) moulted to nymphs with a pre-moulting period of 5.6(±1.7) days. None of the larvae maintained at 10 ± 1 °C moulted. Larval survival was significantly higher at 28 ± 1 °C (44.2 ± 4.3 days) than at 22 ± 1 °C (35.6 ± 9 days). At 28 ± 1 °C, majority of nymphs (95.6%) weighing over 10 mg moulted into adults within 12.5(±3.1) days. Nymphs kept at 10 ± 1 °C underwent diapause for 81.1(±72.0) days. Larger females (>13 mg; 93.0%) laid eggs for of 44.6(±17.8) days with a mean pre-oviposition period of 10.3(±5.1) days. Females survived longer (369.8 ± 128.3 days) than males (210.4 ± 54.1 days; Student's t-test; t = 5.9, p = 0.001). Some females laid eggs without mating. Otobius megnini successfully completed the life cycle within 123 days and has only one nymphal instar similar to the Neotropical population. Currently, O. megnini infestation is a serious problem to horses in the Nuwara Eliya racecourse and may pose a risk of spreading to dairy farms close by owing to its dynamic nature of the life cycle.

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