کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5672907 | 1593431 | 2017 | 5 صفحه PDF | دانلود رایگان |
- Genotyping of Six HCV types using single PCR primer set.
- Genotyping of HCV 1a, 1b, 2, 3, 4, and 6 (6a, 6f, 6i, and 6n) in 30Â min.
- The sensitivity and specificity of the test were 99.1% and 99.7%, respectively.
- 6 HCV Genotyping 9G test can detect mixed genotype infections.
- 6 HCV Genotyping 9G test is a reliable, sensitive, and accurate diagnostic tool.
According to EASL guidelines and WHO recommendations, the accurate detection of HCV genotypes such as HCV 1a, HCV1b, HCV 2, HCV 3, HCV 4, and HCV 6 (6a, 6f, 6i, 6n) is crucial for the efficient treatment of hepatitis C. HCV Genotyping 9G test allows simultaneous genotyping of HCV 1a, 1b, 2, 3, 4, and 6 (6a, 6f, 6i, and 6n) in clinical samples in 30 min. The performance of the test was evaluated by comparison with sequence analysis. Serum samples (n = 152) from HCV-infected patients (n = 110) and healthy individuals (n = 42) were processed under blinded codes. The k coefficient (kappa) values indicated high agreement between the HCV Genotyping 9G test and sequencing. The sensitivity and specificity of the test were 99.1% and 99.7%, respectively. The results indicate that HCV Genotyping 9G test is rapid, reliable, sensitive, and accurate for screening and genotyping of HCV in the clinical specimens.
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Journal: Journal of Virological Methods - Volume 246, August 2017, Pages 95-99