کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5672989 1593428 2017 22 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of the efficiency of different newcastle disease virus reverse genetics systems
ترجمه فارسی عنوان
مقایسه عملکرد سیستم های ژنتیک معکوس بیماری ویروسی نیوکاسل
کلمات کلیدی
ویروس بیماری نیوکاسل، معکوس ژنتیک، کارایی بالاتر نجات،
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی
Rescue of negative-sense single-stranded RNA viruses ((−)ssRNA virus), generally requires the handling of a large number of plasmids to provide the virus genome and essential components for gene expression and genome replication. This constraint probably renders reverse genetics of (−)ssRNA virus more complex and less efficient. Some authors have shown that the fewer the plasmids, the more efficient reverse genetics is for segmented RNA virus. However, it is not clear if the same applies for (−)ssRNA, such as Newcastle disease virus (NDV). To address this issue, six variants of NDV reverse genetic systems were established by cloning combinations of NP, P and L genes, mini-genome or full-genome in 4, 3, 2 and 1 plasmid. In terms of mini-genome and full-genome rescue, we showed that only the 2-plasmid system, assembling three support plasmids together, was able to improve the rescue efficiency over that of the conventional 4-plasmid system. These results may help establish and/or improve reverse genetics for other mononegaviruses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 249, November 2017, Pages 111-116
نویسندگان
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