| کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
|---|---|---|---|---|
| 5673091 | 1593434 | 2017 | 4 صفحه PDF | دانلود رایگان |
کلمات کلیدی
جدول 1- تیتراسیون ویروس CVS پس از تیمار با Virkon S یک درصد
جدول 2- تیتراسیون ويروس CVS پس از تیمار با ايزوپروپيل الكل يا اتانول.
جدول 3- تیتراسیون نمونه های تیمار شده با پارا فرمالدهید.
جدول 4- MIT با نمونه های تیمار شده با PFA و شاهدهای تیمار نشده
- Virkon S (1%) inactivates rabies virus within 1Â min.
- Isopropyl alcohol (70%) is suitable for routine surface decontamination.
- Paraformaldehyde (3% or 4%) treatment for 30Â min is appropriate for fixing cells and inactivates rabies virus.
Rabies virus is a notifiable pathogen that must be handled in high containment facilities where national and international guidelines apply. For the effective inactivation of rabies virus, a number of reagents were tested. Virkon S (1%) solution caused more than 4Â log reduction of rabies virus in culture medium supplemented with 10% foetal calf serum within 1Â min. Isopropyl alcohol (70%) treatment resulted in >3Â log reduction of rabies virus within 20Â s when applied at a ratio of 19:1, making it a suitable agent for surface decontamination whereas 70% ethanol was ineffective. Rabies virus (from 102.33 to 103Â ffu/ml) was also inactivated when cell cultures were fixed with 3% or 4% paraformaldehyde for 30Â min. Regardless of inactivation procedure, when taking inactivated virus preparations out of a biological containment envelope, proof of inocuity must be demonstrated to cover any possible error/deviation from procedure.
Journal: Journal of Virological Methods - Volume 243, May 2017, Pages 109-112