کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5673093 | 1593434 | 2017 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: An improved gold nanoparticle probe-based assay for HCV core antigen ultrasensitive detection An improved gold nanoparticle probe-based assay for HCV core antigen ultrasensitive detection](/preview/png/5673093.png)
- A modified bio-barcode assay for sensitive detection of HCV core antigen.
- Signal DNA on the immuno-complex was detected by TaqMan probe based real-time PCR.
- A detection limit of 1Â fg/mL was measured for HCV core antigen.
A gold nanoparticle probe-based assay (GNPA) was developed for ultrasensitive detection of Hepatitis C virus (HCV) core antigen. In the GNPA, after anti-HCV core antigen polyclonal antibodies and single-stranded barcode signal DNA were labeled on gold nanoparticle probe (NP), DNA enzyme was used to degrade the unbound barcode DNAs. The anti-HCV core antigen monoclonal antibodies were coated on magnetic microparticles probe (MMP). Then the NP-HCV core antigen-MMP sandwich immuno-complex was formed when the target antigen protein was added and captured. Magnetically separated, the immuno-complex containing the single-stranded barcode signal DNA was characterized by TaqMan probe based real-time fluorescence PCR. A detection limit of 1 fg/ml was determined for the HCV core antigen which is magnitude greater than that of ELISA (2Â ng/ml). The coefficients of variation (CV) of intra-assay and inter-assay respectively ranged from 0.22-2.62% and 1.92-3.01%. The improved GNPA decreased the interference of unbound barcode DNAs and may be an new way for HCV core antigen detection.
Journal: Journal of Virological Methods - Volume 243, May 2017, Pages 142-145