کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5673535 | 1593677 | 2017 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The pathogenicity and biological features of Santee-Cooper Ranaviruses isolated from Chinese perch and snakehead fish
ترجمه فارسی عنوان
پاتوژنز و ویژگی های زیست شناختی راینویروس های سانتای کوپر جدا شده از ماهیان چینی و ماهی مار ماهی
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروب شناسی
چکیده انگلیسی
Ranavirus has become a noticeable threat to both farmed and natural populations of fish and amphibians. Herein, we reported that 3 strains of novel viruses, designated as ScRIV-GM-20150902, CmRIV-XT-20150917 and ScRIV-ZS-20151201, were isolated from diseased Chinese perch and snakehead fish in China. Efficient propagation of these isolates were determined in Chinese perch brain (CPB) cell line by the means of cytopathic effect observation, PCR amplification and electron microscopy observation. And their viral titers in CPB cells reached 108.13 TCID50 mlâ1, 107.71 TCID50 mlâ1 and 107.94 TCID50 mlâ1, respectively. While the challenge experiment results showed that 3 isolates resulted in 100% mortality of Chinese perch after virus infection. Electron microscopy analysis showed that two kinds of viral inclusion bodies (intracytoplasmic and intranuclear inclusion body) were observed in infected CPB cells. Sequence alignment and phylogenetic analysis of major capsid protein gene sequences of isolates revealed that these isolates belonged to the species Santee-Cooper Ranavirus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 112, November 2017, Pages 269-273
Journal: Microbial Pathogenesis - Volume 112, November 2017, Pages 269-273
نویسندگان
Xiaozhe Fu, Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang, Ningqiu Li,