کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5673672 | 1593679 | 2017 | 13 صفحه PDF | دانلود رایگان |
- The histone-like protein (hupB) gene of M. tuberculosis was targeted by using two primer sets.
- By Drug Proportion method, the highest percentage (37%) was found resistant to only RIF.
- By MGIT method, the highest percentage (82.2%) was resistant with the triple combination of drugs.
- The highest mutations (76.92%) were found with gene rpoB (531).
- By MAS-PCR mutation in gene embB (306) caused EMB resistance (51.64%).
The various aspects of MDR-TB, type of pathogen, different drug sensitive methods and mutation (s) in specific genes were determined. The histone-like protein (hupB) gene of M. tuberculosis was targeted by using primer sets: N & S and M & S (produced 645 bp & 318 bp fragment respectively). The most significant risk factors were the poverty and male gender of ages 11-25 years. All samples were detected as M. tuberculosis. By Drug Proportion method, the highest percentage (37%) was found resistant to only Rifampin. By MGIT method, the highest percentage (82.2%) was found resistant with the triple combination (Rifampin-RIF + Isoniazid-INH + Ethambutol-EMB) of the drugs. The highest mutations (76.92%) were found in gene rpoB (codon 531) in MDR TB patients. By, MAS-PCR, the highest percentage (34%) were found resistant to combination (INH + RIF) of the drugs. Minimum samples were resistant to RIF and RIF + INH drugs by MGIT, while proportionate results were observed from MAS-PCR and DP. Moreover, by MAS-PCR mutation in gene embB (306) caused EMB resistance (51.64%). We found that M. tuberculosis was the main cause of MDR-TB. Our findings may further be used for an early diagnosis of multi-drug resistant tuberculosis.
Journal: Microbial Pathogenesis - Volume 110, September 2017, Pages 262-274