کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5673879 1593680 2017 22 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct bacterial loop-mediated isothermal amplification detection on the pathogenic features of the nosocomial pathogen - Methicillin resistant Staphylococcus aureus strains with respiratory origins
ترجمه فارسی عنوان
شناسایی تکثیر ایزوترمال متصل به حلقه باکتری مستقیم بر ویژگی های بیماریزای پاتوژن بیمارستانی - سویه های استافیلوکوک اورئوس مقاوم به متیسیلین با ریشه های تنفسی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
چکیده انگلیسی
Loop-mediated isothermal amplification based detection assays using bacterial culture or colony for direct detection of methicillin resistant Staphylococcus aureus(MRSA) had been developed and evaluated, followed by its extensive application on a large scale of clinical MRSA isolated from respiratory origins, including nasal swabs and sputums. Six primers, including outer primers, inner primers and loop primers, were specifically designed for recognizing eight distinct sequences on four targets: 16SrRNA, femA, mecA and orfX. Twenty-seven reference strains were used to develop, evaluate and optimize this assay. Then, a total of 532 clinical MRSA isolates were employed for each detected targets. And the results were determined through both visual observation of the color change by naked eye and electrophoresis. The specific of each primer had been confirmed, and the optimal amplification was obtained under 65 °C for 40 min. The limit of detections (LOD) of bacteria culture LAMP assays were determined to be 104 CFU/ml for 16S rRNA, femA, as well as orfX and 105 CFU/ml for mecA, respectively. The established novel assays on MRSA detection may provide new strategies for rapid detection of foodborne pathogens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 109, August 2017, Pages 183-188
نویسندگان
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