کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5674906 | 1594206 | 2017 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunogenicity of ORFV-based vectors expressing the rabies virus glycoprotein in livestock species
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The parapoxvirus Orf virus (ORFV) encodes several immunomodulatory proteins (IMPs) that modulate host-innate and pro-inflammatory responses and has been proposed as a vaccine delivery vector for use in animal species. Here we describe the construction and characterization of two recombinant ORFV vectors expressing the rabies virus (RABV) glycoprotein (G). The RABV-G gene was inserted in the ORFV024 or ORFV121 gene loci, which encode for IMPs that are unique to parapoxviruses and inhibit activation of the NF-κB signaling pathway. The immunogenicity of the resultant recombinant viruses (ORFVâ024RABV-G or ORFVâ121RABV-G, respectively) was evaluated in pigs and cattle. Immunization of the target species with ORFVâ024RABV-G and ORFVâ121RABV-G elicited robust neutralizing antibody responses against RABV. Notably, neutralizing antibody titers induced in ORFVâ121RABV-G-immunized pigs and cattle were significantly higher than those detected in ORFVâ024RABV-G-immunized animals, indicating a higher immunogenicity of ORFVÎ121-based vectors in these animal species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 511, November 2017, Pages 229-239
Journal: Virology - Volume 511, November 2017, Pages 229-239
نویسندگان
Mathias Martins, Lok R. Joshi, Fernando S. Rodrigues, Deniz Anziliero, Rafael Frandoloso, Gerald F. Kutish, Daniel L. Rock, Rudi Weiblen, Eduardo F. Flores, Diego G. Diel,