کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5675034 | 1594209 | 2017 | 9 صفحه PDF | دانلود رایگان |
- Regulation of downstream gene expression at NiV gene junctions is not constant.
- The 3â² non-coding regions are responsible for reducing of downstream gene expression.
- Uridine-rich tracts within the L 3â² NCR signal transcription termination.
The regulation of transcription during Nipah virus (NiV) replication is poorly understood. Using a bicistronic minigenome system, we investigated the involvement of non-coding regions (NCRs) in the transcriptional re-initiation efficiency of NiV RNA polymerase. Reporter assays revealed that attenuation of NiV gene expression was not constant at each gene junction, and that the attenuating property was controlled by the 3â² NCR. However, this regulation was independent of the gene-end, gene-start and intergenic regions. Northern blot analysis indicated that regulation of viral gene expression by the phosphoprotein (P) and large protein (L) 3â² NCRs occurred at the transcription level. We identified uridine-rich tracts within the L 3â² NCR that are similar to gene-end signals. These gene-end-like sequences were recognized as weak transcription termination signals by the viral RNA polymerase, thereby reducing downstream gene transcription. Thus, we suggest that NiV has a unique mechanism of transcriptional regulation.
Journal: Virology - Volume 508, August 2017, Pages 36-44