کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5675380 | 1594319 | 2017 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of a novel linear B-cell epitope in the p27 of Avian leukosis virus
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
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چکیده انگلیسی
Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can induce virus-associated neoplasia and causes great economic loss in poultry industry. It is known that the capsid antigen p27 is the group-specific antigen that is highly conserved among all ALV subgroups, and is the most abundant immunogenic viral protein. In the present study, five overlapping fragments (GST- p27-F1/2, GST- p27-F2-1/2/3) of ALV-p27 were subjected to Western blotting analysis using a monoclonal antibody (5D3) against ALV-p27 to identify the epitope. The result showed that the epitope recognized by 5D3 is located within 173-240 amino acid of the ALV-p27 protein. For precise mapping of this epitope, a set of overlapping peptides were synthesized. Indirect enzyme linked immunosorbent assay (ELISA) revealed that 193CFRQKSQPDI202 motif was the minimal fragment recognized by 5D3, so this motif represented a linear B-cell epitope of ALV-p27. Homology analysis indicated that 5D3 defined epitope is highly conserved among ALV strains. The identified epitope might be useful in clinical applications and as a tool for further study of the structure and function of ALV-p27.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 238, 15 June 2017, Pages 253-257
Journal: Virus Research - Volume 238, 15 June 2017, Pages 253-257
نویسندگان
Wiaam O.A. Khairy, Lin Wang, Xue Tian, Jianqiang Ye, Kun Qian, Hongxia Shao, Aijian Qin,