کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5675437 1594329 2017 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evidence of genotypes 1 and 3 of avian hepatitis E virus in wild birds
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Evidence of genotypes 1 and 3 of avian hepatitis E virus in wild birds
چکیده انگلیسی
Although the presence of four genotypes of avian hepatitis E virus (HEV) in chickens has been demonstrated, its natural host range is still barely known. In this study, swab samples from 626 wild birds originating from 62 bird species were investigated for HEV detection by molecular methods. The aim was to explore the cross-species infection of avian HEV and to compare the genetic diversity between strains infecting chicken and wild birds. In total, 8 positive samples from 4 different bird species (song thrush, little owl, feral pigeon and common buzzard) were identified and further confirmed by partial sequencing of ORF3. Based on a 237 bp fragment of the capsid gene retrieved from 5 samples, phylogenetic analysis revealed the presence of avian HEV genotypes 1 and 3 in wild birds. The wild bird isolates shared 82.7-84.8% and 85.7-100% nucleotide sequence identity, respectively, to chicken isolates from the corresponding genotype. For two of the genotype 1 samples (14-2901 and 14-2906), from feral pigeons, genotype assignment could be also confirmed by phylogenetic analysis based on partial nucleotide sequence of the helicase gene. For the first time, the appearance of genotype 1 in Europe was detected, which together with close genetic relationship between HEVs present in chickens and wild birds indicates cross-species transmission.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 228, 15 January 2017, Pages 75-78
نویسندگان
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