کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5685731 1598521 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats
ترجمه فارسی عنوان
جایگزینی تستوسترون، محتوای عضلانی صاف را در کورنووسوم کروی موش های ارکیکتومیز نگه می دارد
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی بیماری‌های کلیوی
چکیده انگلیسی

ObjectiveTo evaluate the effects of testosterone (T) on the maintenance of corpus cavernosum (CC) structure and apoptosis.MethodsAnimals were divided into three groups: sham operation group (n = 8) underwent sham operation; Orchiectomized (Orchiec)+ oily vehicle group (n = 8) underwent bilateral orchiectomy and received a single dose of oily vehicle by intramuscular injection (i.m.) 30 days after orchiectomy; and Orchiec + T group (n = 8) underwent bilateral orchiectomy and received a single dose of T undecanoate 100 mg/kg i.m. 30 days after the surgery. Animals were euthanized 60 days after the beginning of the experiment with an anesthetic overdose of ketamine and xylazine. Blood samples and penile tissue were collected on euthanasia. Azan's trichrome staining was used to evaluate smooth muscle, Weigert's Fucsin-Resorcin staining was used to evaluate elastic fibers and Picrosirius red staining was used to evaluate collagen. Apoptosis was evaluated using TUNEL technique.ResultsT levels decreased in Orchiec + oily vehicle when compared to sham operation and Orchiec + T groups (p < 0.001). T deprivation reduced trabecular smooth muscle content and penile diameter and T replacement maintained both parameters (p = 0.005 and p = 0.001, respectively). No difference was observed in the content of sinusoidal space (p = 0.207), elastic fibers (p = 0.849), collagen (p = 0.216) and in apoptosis (p = 0.095).ConclusionNormal testosterone levels maintain CC smooth muscle content and do not influence elastic fibers, collagen content and apoptotic index. Further studies should be performed in order to investigate the mechanisms by which androgen mediates its effects on CC structure.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Asian Journal of Urology - Volume 4, Issue 4, October 2017, Pages 223-229
نویسندگان
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