کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5735511 | 1411868 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Region-specific increases in FosB/ÎFosB immunoreactivity in the rat brain in response to chronic sleep restriction
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کلمات کلیدی
NREMAllostasisexercise controlMCHREMrecABCCSRγ-aminobutyric acid - اسید γ-آمینوبوتیریکimmunoreactive - ایمنی فعالImmunohistochemistry - ایمونوهیستوشیمیanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancerapid eye movement - حرکت سریع چشمnon-rapid eye movement - حرکت سریع چشم نیستstandard error of the mean - خطای استاندارد میانگینRecovery - ریکاوریHome cage - قفس خانهchronic sleep restriction - محدودیت خواب مزمنSleep deprivation - محرومیت از خوابSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیmelanin-concentrating hormone - هورمون متمرکز کننده ملانینGABA - گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب رفتاری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Using a rat model of chronic sleep restriction (CSR) featuring periodic sleep deprivation with slowly rotating wheels (3Â h on/1Â h off), we previously observed that 99Â h of this protocol induced both homeostatic and allostatic (adaptive) changes in physiological and behavioural measures. Notably, the initial changes in sleep intensity and attention performance gradually adapted during CSR despite accumulating sleep loss. To identify brain regions involved in these responses, we used FosB/ÎFosB immunohistochemistry as a marker of chronic neuronal activation. Adult male rats were housed in motorized activity wheels and underwent the 3/1 CSR protocol for 99Â h, or 99Â h followed by 6 or 12Â days of recovery. Control rats were housed in home cages, locked activity wheels, or unlocked activity wheels that the animals could turn freely. Immunohistochemistry was conducted using an antibody that recognized both FosB and ÎFosB, and 24 brain regions involved in sleep/wake, autonomic, and limbic functions were examined. The number of darkly-stained FosB/ÎFosB-immunoreactive cells was increased immediately following 99Â h of CSR in 8/24 brain regions, including the medial preoptic and perifornical lateral hypothalamic areas, dorsomedial and paraventricular hypothalamic nuclei, and paraventricular thalamic nucleus. FosB/ÎFosB labeling was at control levels in all 8 brain areas following 6 or 12 recovery days, suggesting that most of the immunoreactivity immediately after CSR reflected FosB, the more transient marker of chronic neuronal activation. This region-specific induction of FosB/ÎFosB following CSR may be involved in the mechanisms underlying the allostatic changes in behavioural and physiological responses to CSR.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Behavioural Brain Research - Volume 322, Part A, 30 March 2017, Pages 9-17
Journal: Behavioural Brain Research - Volume 322, Part A, 30 March 2017, Pages 9-17
نویسندگان
Shannon Hall, Samüel Deurveilher, Kristin Robin Ko, Joan Burns, Kazue Semba,