کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5737760 | 1614732 | 2017 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Direct effects of glucose, insulin, GLP-1, and GIP on bulbospinal neurons in the rostral ventrolateral medulla in neonatal wistar rats
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کلمات کلیدی
GLUTGLP-1RSNAaCSFGIPTTXIMLFAPRVLMGIPRGLP-1rostral ventrolateral medulla - آرام آرامinsulin - انسولینtetrodotoxin - تترو دوتوکسین Glucose transporter - حمل و نقل گلوکزintermediolateral cell column - ستون سلول میان سلولیHypertension - فشار خون بالاBlood pressure - فشارخونartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیaction potential - پتانسیل عمل Membrane potential - پتانسیل غشاء Glucose - گلوکزinsulin receptor - گیرنده انسولینglucagon-like peptide-1 receptor - گیرنده پپتید-1 مانند گلوکاگون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Although patients with diabetes mellitus (DM) often exhibit hypertension, the mechanisms responsible for this correlation are not well known. We hypothesized that the bulbospinal neurons in the rostral ventrolateral medulla (RVLM) are affected by the levels of glucose, insulin, or incretins (glucagon like peptide-1 [GLP-1] or glucose-dependent insulinotropic peptide [GIP]) in patients with DM. To investigate whether RVLM neurons are activated by glucose, insulin, GLP-1, or GIP, we examined changes in the membrane potentials of bulbospinal RVLM neurons using whole-cell patch-clamp technique during superfusion with various levels of glucose or these hormones in neonatal Wistar rats. A brainstem-spinal cord preparation was used for the experiments. A low level of glucose stimulated bulbospinal RVLM neurons. During insulin superfusion, almost all the RVLM neurons were depolarized, while during GLP-1 or GIP superfusion, almost all the RVLM neurons were hyperpolarized. Next, histological examinations were performed to examine transporters for glucose and receptors for insulin, GLP-1, and GIP on RVLM neurons. Low-level glucose-depolarized RVLM neurons exhibited the presence of glucose transporter 3 (GLUT3). Meanwhile, insulin-depolarized, GLP-1-hyperpolarized, and GIP-hyperpolarized RVLM neurons showed each of the respective specific receptor. These results indicate that a low level of glucose stimulates bulbospinal RVLM neurons via specific transporters on these neurons, inducing hypertension. Furthermore, an increase in insulin or a reduction in incretins may also activate the sympathetic nervous system and induce hypertension by activating RVLM neurons via their own receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 344, 6 March 2017, Pages 74-88
Journal: Neuroscience - Volume 344, 6 March 2017, Pages 74-88
نویسندگان
Naoki Oshima, Hiroshi Onimaru, Hidehito Matsubara, Takahiro Uchida, Atsushi Watanabe, Toshihiko Imakiire, Yasuhiro Nishida, Hiroo Kumagai,