کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5737900 | 1614725 | 2017 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Label-free quantitative proteomics unravels the importance of RNA processing in glioma malignancy
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کلمات کلیدی
GBMTNRFTH1STRINGRab3AMAPK1LGAEGFRNCLSNCAFGF1LAMC1Syt1cancer pathogenesisMALDI-TOFFDRSynaptotagmin IDTTLC-MS/MS - LC-MS / MSStat1 - sTAT1Low-grade astrocytoma - آستروسیتوما درجه پایینSearch Tool for the Retrieval of Interacting Genes - ابزار جستجو برای بازیابی ژن های تعاملKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو dithiothreitol - دیتیوتریتولFerritin Heavy Chain - زنجیره سنگین فریتینWorld Health Organization - سازمان بهداشت جهانیMass spectrometry - طیف سنجی جرمیFibroblast growth factor 1 - عامل رشد فیبروبلاست 1signal transducer and activator of transcription 1 - مبدل سیگنال و فعال کننده رونویسی 1Matrix-assisted laser desorption/ionization time of flight mass spectrometry - مدت زمان جذب / زمان یونیزاسیون طیف سنجی جرمی پرواز با کمک ماتریکسLabel-free quantification - مقدار کافی تعیین نشدهfalse discovery rate - میزان کشف کاذبNeurocan - نوروکانNucleolin - نولولینmitogen-activated protein kinase 1 - پروتئین کیناز فعال با mitogen-1liquid chromatography tandem mass spectrometry - کروماتوگرافی مایع اسپکترومتری دو طرفهhigh-pressure liquid chromatography - کروماتوگرافی مایع با فشار بالاHPLC - کروماتوگرافی مایعی کاراWHO - کهGlioblastoma - گلیوبلاستوماEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمالAgrin - یک نیشخند
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Glioma, one of the most common cancers in human, is classified to different grades according to the degrees of malignancy. Glioblastoma (GBM) is known to be the most malignant (Grade IV) whereas low-grade astrocytoma (LGA, Grade II) is relatively benign. The mechanism underlying the pathogenesis and progression of glioma malignancy remains unclear. Here we report a quantitative proteomic study to elucidate the differences between GBM and LGA using liquid chromatography and tandem mass spectrometry followed by label-free quantification. A total of 136 proteins were differentially expressed in GBM for at least five folds in comparison with LGA. Ontological analysis revealed a close correlation between GBM-associated proteins and RNA processing. Interaction network analysis indicated that the GBM-associated proteins in the RNA processing were linked to crucial signaling transduction modulators including epidermal growth factor receptor (EGFR), signal transducer and activator of transcription 1 (STAT1), and mitogen-activated protein kinase 1 (MAPK1), which were further connected to the proteins important for neuronal structural integrity, development and functions. Upregulation of 40S ribosomal protein S5 (RPS5), Ferritin Heavy chain (FTH1) and STAT1, and downregulation of tenascin R (TNR) were validated as representatives by immune assays. In summary, we revealed a panel of GBM-associated proteins and the important modulators centered at the RNA-processing network in glioma malignancy that may become novel biomarkers and help elucidate the underlying mechanism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 351, 20 May 2017, Pages 84-95
Journal: Neuroscience - Volume 351, 20 May 2017, Pages 84-95
نویسندگان
Baibin Bi, Feng Li, Jisheng Guo, Cuiling Li, Ruirui Jing, Xin Lv, Xinjun Chen, Fengqin Wang, Kazem M. Azadzoi, Lin Wang, Yuguang Liu, Jing-Hua Yang,