کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5738211 | 1615048 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Astrocytic expression of the RNA regulator HuR accentuates spinal cord injury in the acute phase
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کلمات کلیدی
GFAPBMSRBPSpinal cord injury - آسیب نخاعیSpinal cord injury (SCI) - آسیب نخاعی (SCI)Transgenic - تراریختهsci - علمیBlood spinal cord barrier - مانع طناب نخاعی خونPost-transcriptional regulation - مقررات پست مدرنBasso Mouse Scale - مقیاس ماوس پایینGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالRNA binding protein - پروتئین متصل به RNAHuR - چگونه
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We recently showed that the RNA regulator, HuR, is translocated to the cytoplasm in astrocytes in the acute phase of spinal cord injury (SCI), consistent with its activation. HuR positively modulates expression of many pro-inflammatory factors, including IL-1β, TNF-α, and MMP-12, which are present at high levels in the early phase of SCI and exacerbate tissue damage. Knockdown of HuR in astrocytes blunts expression of these factors in an in vitro stretch injury model of CNS trauma. In this report, we further investigate the impact of HuR in early SCI using a mouse model in which human HuR is transgenically expressed in astrocytes. At 24 h following a mid-thoracic contusion injury, transgenic HuR translocated to the cytoplasm of astrocytes, similar to endogenous HuR, and consistent with its activation. Compared to littermate controls, the transgenic mice showed a global increase in astrocyte activation at the level of injury and a concomitant increase in vascular permeability. There was a significant decrease in neuronal survival at this time interval, but no differences in white matter sparing. Long term behavioral assessments showed no difference in motor recovery. In summary, transgenic expression of HuR in astrocytes accentuated neuronal injury and other secondary features of SCI including increased vascular permeability and astrocyte activation. These findings underscore HuR as a potential therapeutic target in early SCI.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 651, 9 June 2017, Pages 140-145
Journal: Neuroscience Letters - Volume 651, 9 June 2017, Pages 140-145
نویسندگان
Thaddaeus Kwan, Candace L. Floyd, Jason Patel, Amanda Mohaimany-Aponte, Peter H. King,