کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5740282 1616294 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analyses of 16S rRNA and cpn60 gene sequences provide complementary information about potentially useful and harmful oil field microbiota
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست علوم زیست محیطی (عمومی)
پیش نمایش صفحه اول مقاله
Analyses of 16S rRNA and cpn60 gene sequences provide complementary information about potentially useful and harmful oil field microbiota
چکیده انگلیسی


- Microbes implicated in corrosion and biosurfactant production were detected.
- Discrepancies were found in results of 16S rRNA and cpn60 gene sequence analyses.
- Simultaneous targeting of 16S rRNA and cpn60 genes may mitigate PCR biases.

Microbial communities from five wells in a Canadian oil field were characterized by sequencing 16S rRNA and cpn60 gene amplicons. Analysis of 16S rRNA amplicon sequences using the RDPipeline and the Phoenix 2 pipeline showed that amplicons representing Ralstonia, Arcobacter, Sulfurospirillum, Methanohalophilus, Methanocalculus and Methanolobus spp. were abundant in most samples. Amplicons derived from Bacillus and some other genera were detected in samples as well. Analysis of cpn60 sequences confirmed that amplicons derived from Sulfurospirillum, Methanolobus and Bacillus spp. were well represented, and showed that a bacterium highly similar to biosurfactant producing Bacillus mojavensis represented the genus Bacillus. In addition, analysis of cpn60 sequences showed that amplicons derived from the Deltaproteobacteria, particularly Pelobacter carbinolicus, were abundant. However, analysis of cpn60 sequences failed to detect amplicons representing Ralstonia spp. or other representatives of the Betaproteobacteria, indicating possible PCR bias. Our findings suggest that gaps resulting from shortcomings of 16S rRNA and cpn60 gene amplification and analytical systems can be overcome through simultaneous targeting of these conserved genes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Biodeterioration & Biodegradation - Volume 123, September 2017, Pages 320-327
نویسندگان
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