کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5740420 | 1616296 | 2017 | 9 صفحه PDF | دانلود رایگان |
- Both TUAH-1Â cell and its crude enzymes have capacity of CTN degradation in both aqueous and soil.
- After treatment with 21.3 mg dry cells, the degradation efficiency was 97.4% for 20 mg L-1 CTN in the aqueous phase.
- Treatment with crude enzyme solution containing 2.0 mg mL-1 protein led to complete degradation of 20.0 mg L-1 CTN within 10 h, which is significantly faster than that of TUAH-1.
- According to degradation products detected by HPLC-MS, degradation pathways of CTN by TUAH-1 were proposed.
- The mechanism of CTN degradation by TUAH-1 is multi-enzymatic catalysis.
The long-term excessive use of chlorothalonil (CTN) can lead to serious environmental pollution, which presents a cause for concern. Here, we investigated the degradation of CTN by using an effective strain, TUAH-1, which was identified as Enterobacter cloacae according to the morphological approach, 16S rDNA gene sequence and phylogenetic tree analysis. Treatment with TUAH-1 demonstrated that the maximum processing capability was 74 mg CTN per gram dry cells in 48 h under optimum conditions (30 °C-35 °C, pH 7.0). After treatment with 21.3 mg dry cells, the degradation efficiency was 97.4% for 20 mg l-1 CTN in the aqueous phase. Meanwhile, treatment with crude enzyme solution containing 2.0 mg ml-1 protein led to complete degradation of 20.0 mg l-1 CTN within 10 h, which is significantly faster than that of TUAH-1. The results also showed that CTN could not be detected after treatment with 5.68 g TUAH-1 per kg soil for 48 h when the original CTN content in the soil was 10 mg kg-1. After treatment with THAH-1 or its crude proteins, many degradation products were detected by HPLC-MS, suggesting two possible degradation pathways. The mechanism of CTN degradation by TUAH-1 was noted to be multienzymatic catalysis comprising glyceraldehyde-3-phosphate dehydrogenase and glutathione S-transferase.
Journal: International Biodeterioration & Biodegradation - Volume 121, July 2017, Pages 122-130