کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5740939 1616541 2017 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Study of Streptococcus thermophilus population on a world-wide and historical collection by a new MLST scheme
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Study of Streptococcus thermophilus population on a world-wide and historical collection by a new MLST scheme
چکیده انگلیسی


- A world-wide population scheme of S. thermophilus
- A phylogenetic analysis in agreement with whole-genome analysis
- Evidence for association between groups and geographical origin
- Persistence of S. thermophilus in cheese making sites
- Impact of starter use on S. thermophilus population in traditional cheeses

We analyzed 178 Streptococcus thermophilus strains isolated from diverse products, from around the world, over a 60-year period with a new multilocus sequence typing (MLST) scheme. This collection included isolates from two traditional cheese-making sites with different starter-use practices, in sampling campaigns carried out over a three years period. The nucleotide diversity of the S. thermophilus population was limited, but 116 sequence types (ST) were identified. Phylogenetic analysis of the concatenated sequences of the six housekeeping genes revealed the existence of groups confirmed by eBURST analysis. Deeper analyses performed on 25 strains by CRISPR and whole-genome analysis showed that phylogenies obtained by MLST and whole-genome analysis were in agreement but differed from that inferred by CRISPR analysis. Strains isolated from traditional products could cluster in specific groups indicating their origin, but also be mixed in groups containing industrial starter strains. In the traditional cheese-making sites, we found that S. thermophilus persisted on dairy equipment, but that occasionally added starter strains may become dominant. It underlined the impact of starter use that may reshape S. thermophilus populations including in traditional products. This new MLST scheme thus provides a framework for analyses of S. thermophilus populations and the management of its biodiversity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 242, 2 February 2017, Pages 70-81
نویسندگان
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