کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5745403 | 1618662 | 2017 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Original articleIn vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with Androcoll-E⢠Original articleIn vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with Androcoll-Eâ¢](/preview/png/5745403.png)
A colloid with a species specific silane-coated, silica-based formulation, optimized for stallion (Androcoll-Eâ¢), enables a better sub-population of spermatozoa to be selected from stallion ejaculates. However, such a practice has not been critically evaluated in stallions with fertility problems. In this study we evaluate whether single-layer centrifugation (SLC) through Androcoll-E⢠could be used to enhance fertility rates in a subfertile stallion. Ejaculates were obtained from two different stallions, one Lusitano (fertile) and one Sorraia (subfertile), with distinct sperm characteristics and fertility. Motility, morphology, plasma membrane structural (eosin-nigrosin) and functional integrity (HOS test), mitochondrial functionality (ÎÏm; JC-1) and longevity (motility after 72 h cooling) after centrifugation in Androcoll-Eâ¢, as well as pregnancy rates obtained after artificial insemination (AI), with and without (control group) SLC-treated sperm were assessed. The effect of SLC on sperm characteristics, and fertility results were evaluated by ANOVA and Fisher procedures, respectively. Our results showed that SLC-selected sperm did not differ from the raw semen in terms of viability, morphology, response to hypo-osmotic conditions (HOS test) and mitochondrial membrane potential (âÎΨmit; JC-1). Sperm motility in cooled samples was not improved by SLC treatment. Our data show that SLC through Androcoll-E⢠has no effect on pregnancy rates in the stallions used in this trial.
Journal: Saudi Journal of Biological Sciences - Volume 24, Issue 7, November 2017, Pages 1489-1496