Seasonal variations of carbonic anhydrase activity in Chongqing urban section of Jialing River and its influencing factors

Carbonic anhydrase (CA) is an enzyme in algal carbon-utilization that plays an important role in the formation of algal blooms. A year-long monitoring program in the shore area of Chongqing Urban Section of the Jialing River (JR) was launched to determine the variations in carbonic anhydrase activity (CAA) and its change mechanism in the hydro-fluctuation belt of the tributaries in the Three Gorges Reservoir (TGR) area. The variations in basic water quality parameters, different carbon forms, and CAA were investigated from November 2013 to October 2014. Results showed that the mean CAA value in JR was 0.67 ± 0.31 EU/106 cells. CAA was high during the flood stage, low during the impounding stage, and peaked on April 3, 2014 during the discharging stage. No significant difference was observed in the CAA of different sampling sites in JR. However, a significant difference was observed between the CAA of JR and that of the Yangtze River. Correlation analyses showed that water temperature, pH, algal cell density, and dissoluble organic carbon were positively correlated with CAA, whereas CO2 and dissoluble inorganic carbon were negatively correlated with CAA. A model for CAA and related parameters was built through principal component regression. The equation was expressed as follows: CAA = 0.116T + 0.00746Cells+0.0156pH−0.0157CO2−0.0150DIC+0.0135DOC+0.565. Results revealed that CAA in JR was controlled by multiple factors, which could be used for CAA monitoring. The model demonstrated a potential value in controlling algal blooms.