کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5747628 1618922 2017 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of cell-based assays for toxicity characterization of complex wastewater matrices: Possible applications in wastewater recycle and reuse
ترجمه فارسی عنوان
استفاده از آزمایشات مبتنی بر سلول برای توصیف سمیت ماتریس های فاضلاب پیچیده: کاربرد های احتمالی در بازیافت و استفاده مجدد فاضلاب
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
چکیده انگلیسی


- Wastewater recycling is an effective tool to meet freshwater demand.
- Toxicity assessment of recycled wastewater is equally important.
- Biochemical and analytical methods have their own limitations.
- We propose cell-based assays for toxicity characterization of wastewater matrices.
- They can be used for monitoring wastewater treatment and recycling processes efficacy.

Exposure to pre-concentrated inlet or outlet STP wastewater extracts at different concentrations (0.001% to 1%) induced dose-dependent toxicity in MCF-7 cells, whereas drinking water extracts did not induce cytotoxicity in cells treated. GC-MS analysis revealed the occurrence of xenobiotic compounds (Benzene, Phthalate, etc.) in inlet/outlet wastewater extracts. Cells exposed to inlet/outlet extract showed elevated levels of reactive oxygen species (ROS: inlet: 186.58%, p<0.05, outlet, 147.8%, p<0.01) and loss of mitochondrial membrane potential (Δψm: inlet, 74.91%, p<0.01; outlet, 86.70%, p<0.05) compared to the control. These concentrations induced DNA damage (Tail length: inlet: 34.4%, p<0.05, outlet, 26.7%, p<0.05) in treated cells compared to the control (Tail length: 7.5%). Cell cycle analysis displayed drastic reduction in the G1 phase in treated cells (inlet, G1:45.0%; outlet, G1:58.3%) compared to the control (G1:67.3%). Treated cells showed 45.18% and 28.0% apoptosis compared to the control (1.2%). Drinking water extracts did not show any significant alterations with respect to ROS, Δψm, DNA damage, cell cycle and apoptosis compared to the control. Genes involved in cell cycle and apoptosis were found to be differentially expressed in cells exposed to inlet/outlet extracts. Herein, we propose cell-based toxicity assays to evaluate the efficacies of wastewater treatment and recycling processes.

Possible application of cell-based assays for toxicity characterization of wastewater matrices.252

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 142, August 2017, Pages 555-566
نویسندگان
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