Biosynthesis of gold nanoparticles using yellow oyster mushroom Pleurotus cornucopiae var. citrinopileatus

- A rapid myco-reduction of chloroauric acid to Au nanoparticles using fresh and dried aqueous extracts of yellow oyster mushroom.
- Synthesis of gold nanoparticles was confirmed by FESEM, HRTEM, EDX and FT-IR analyses.
- The gold nanoparticles showed spherical shapes with size ranged from 16 to 100 nm.

In this study, two extracts of Pleurotus cornucopiae var. citrinopileatus mushroom (fresh and dried) were investigated as reducing and capping factors for biosynthesis of gold nanoparticles (AuNPs). In extracellular, AuNPs were synthesized using Chloroauric acid with extracts of mushroom. The incubated solution from gold ions and mushroom's extract under dark condition were reduced to AuNPs. Fresh mushroom extract showed better reduction. The synthesized AuNPs were characterized using color, UV-visible spectroscopy, FTIR, EDX, FESEM, and HRTEM. Purple color after 24 h of incubation was given indication for AuNPs formation. Results showed that AuNP absorption band was located on a peak of 550 nm for a solution from mixing Au ions with fresh mushroom, and 540 nm for mixing Au ions with the dried one. Both the FESEM and HRTEM confirmed that the formation of AuNPs was mainly spherical within the range of 16-91 nm for AuNPs of dried mushroom and 23-100 nm for AuNPs of fresh one.