کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5756167 1622544 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ambient fine particulate matters induce cell death and inflammatory response by influencing mitochondria function in human corneal epithelial cells
ترجمه فارسی عنوان
مسائل جزئی ذرات ریز باعث مرگ سلول و پاسخ التهابی می شود با تأثیر عملکرد میتوکندری در سلول های اپیتلیال قرنیه انسان
کلمات کلیدی
ذرات ریز محیطی، چشم، قرنیه، استرس اکسیداتیو، میتوکندریا،
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
Ambient fine particulate matter (AFP) is a main risk factor for the cornea as ultraviolet light. However, the mechanism of corneal damage following exposure to AFP has been poorly understood. In this study, we first confirmed that AFP can penetrate the cornea of mice, considering that two-dimensional cell culture systems are limited in reflecting the situation in vivo. Then, we investigated the toxic mechanism using human corneal epithelial (HCET) cells. At 24 h after exposure, AFP located within the autophagosome-like vacuoles, and cell proliferation was clearly inhibited in all the tested concentration. Production of ROS and NO and secretion of pro-inflammatory cytokines were elevated in a dose-dependent manner. Additionally, conversion of LC3B from I-type to II-type and activation of caspase cascade which show autophagic- and apoptotic cell death, respectively, were observed in cells exposed to AFP. Furthermore, AFP decreased mitochondrial volume, inhibited ATP production, and altered the expression of metabolism-related genes. Taken together, we suggest that AFP induces cell death and inflammatory response by influencing mitochondrial function in HCET cells. In addition, we recommend that stringent air quality regulations are needed for eye health.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Research - Volume 159, November 2017, Pages 595-605
نویسندگان
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