کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5762499 | 1624889 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polymeric hydrophilic interaction liquid chromatography coupled with Orbitrap mass spectrometry and chemometric analysis for untargeted metabolite profiling of natural rice variants
ترجمه فارسی عنوان
کروماتوگرافی مایع متقاطع هیدروفولی پلیمری همراه با اسپکترومتری جرمی اوربیتپور و تجزیه و تحلیل شیمیایی برای پروفایل متابولیت غیر هدفمند از انواع برنج طبیعی
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
چکیده انگلیسی
GC-MS based metabolomics of genetically modified rice varieties are widely reported but involves tedious derivatization processes. Broad-scale LC-MS-based metabolomics are lacking or limited to targeted analyses. In this study, metabolite diversity in natural rice variants including basmati and non-basmati rice varieties was investigated using polymeric hydrophilic interaction chromatography coupled to an Orbitrap mass analyzer. Identification was performed using exact mass and standard retention times. A total of 329 metabolites were selected using IDEOM (Identification of metabolites) software. Statistical tests were used to filter out top 50 rice varieties discriminating metabolites. Several up- or down-regulated metabolites were observed in rice varieties and can be used as biomarker compounds to differentiate between rice varieties. The developed method and generated data can be used for the comparison of genetically modified rice varieties with natural ones, an important requirement for the implementation of comparator-based GMO risk assessment procedures demanded by national and international regulations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cereal Science - Volume 73, January 2017, Pages 165-173
Journal: Journal of Cereal Science - Volume 73, January 2017, Pages 165-173
نویسندگان
Muhammad Irfan Akram, Isabel M. Vincent, Amna Jabbar Siddiqui, Syed Ghulam Musharraf,